Data from: Molecular and cellular processes of deafferentation after plexus injury in human dorsal root ganglia

Dataset of human dorsal root ganglia (DRG) images and deep learning models

Sections of six control and six plexus patient DRG were immunostained and imaged using large-field (tile) microscopy. Two staining combinations were investigated: 

- neurofilament (NF), fatty acid binding protein 7 (FABP7), and clusterin (APOJ)

- neurofilament (NF), ionized calcium-binding adapter molecule 1 (IBA1), and microtubule-associated protein 2 (MAP2) 

NF-positive neurons were segmented with a deep learning method (deepflash2). FABP7 and IBA1 were segmented with a thresholding method.

Provided are:

- all images and corresponding segmentation masks for NF, FABP7, and IBA1 

- images and annotated segmentation masks of NF-positive neurons (3 experts annotated the same 10 images, the estimated ground truth masks were used for training of deep learning models)

- the deep learning model ensemble (3) for segmentation of NF-positive neurons