Gene Editing of Anp32a Confers Resistance to Avian Influenza in Chicken. Gene Editing of Anp32a Confers Resistance to Avian Influenza in Chicken

Comparison of transcriptomic profiles of primordial germ cells and derived fibroblast cell lines from homozygous, CRISPR/Cas9 gene-edited, ANP32A knockout and mutant chickens Overall design: We used CRISPR/Cas9 to perform precise sequence changes in ANP32A to generate genome-edited PGC cell lines, which were also differentiated into fibroblast-like cell lines, all of which were subjected to RNA-seq in their native state.