Transcriptional changes upon Mphosph8 or Morc2a loss in sorted neuronal nuclei of adult mouse brains.

We report that murine MPP8 and MORC2A are highly expressed in the brain and exclusively found in neurons. We show that genetic inactivation of Mphosph8 (coding for MPP8) or Morc2a in the nervous system of mice leads to alterations in brain architecture, behavioral impairments and reduction of life span. By doing RNA-seq and ChIP-seq experiments, we found that MPP8 and MORC2A suppress the repetitive-like protocadherin gene cluster on mouse chromosome 18 in a H3K9me3-dependent manner. Overall design: Examination of consequences of Nestin-Cre-mediated nervous system-specific knock-out of Mphosph8 and Morc2a in FACS-sorted neuronal nuclei (from at least 2 brains per genotype).