Lysosomal Dynamics Orchestrate Neurogenesis [RIP-Seq]. Lysosomal Dynamics Orchestrate Neurogenesis [RIP-Seq]

Lysosomes are cellular recycling stations and metabolic signaling hubs. Whether lysosome dynamics regulate mammalian brain development is unknown. We found that radial glia cells possess a large number of endolysosomes and that asymmetric inheritance of lysosomes in daughters of radial glia cells can predict fate and cell cycle length. To determine the lysosomal regulation of translation initiation by mTORC1/eIF4E axis, we performed RNA immunoprecipitation sequencing (RIP-seq) with antibody against eIF4E in E13.5 neocortex. Overall design: RIP-seq was performed for cytoplasmic mRNA in mouse E13.5 brain neocortex with eIF4E antibody (MBL, RN001P) or control immunoglobulin G (Bioss, bs0295P).