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mRNA-Seq of placentas bearing the OgtY851A mutation

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Heterozygous OgtY851A/WT females were bred with hemizygous OgtY851A/Y males, placentas were dissected at E12.5 and single placenta's halves were processed for mRNA-Seq. Sex and genotypes of the embryos were determined by PCR genotyping of the DNA extracted from the embryonic heads.

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