Transcriptome changes induced by bitter melon (Momordica charantia)- intake in a high-fat diet induced obesity model

Metabolic effects of lyophilized bitter melon juice (BMJ) extract (oral gavage 200mg/kg/body weight-daily for 40 days) intake were evaluated in diet-induced obese C57BL/6J male mice [fed-high fat diet (HFD), 60 kcal% fat]. Changes in gene expression in the hepatic transcriptome (microarray analysis using Affymetrix Mouse Exon 1.0 ST arrays) Expression estimates for individual genes were estimated using Affymetrix Power Tools and the RMA-sketch algorithm. Probe sets were grouped into gene clusters using Ensembl gene annotation (version GRCm38). A probe set was included in a gene cluster if all of its associated probes aligned to a region completely contained within the exonic region of the gene. Total of 7 samples from mice randomized into two groups: a) Control-DIO group (n = 3 per group) and b) BMJ+DIO group (n = 4 per group)