Primer sequences and predicted amplicon sizes.

BRAF and MAP2K1 target regions are listed with the corresponding primer sequences and 5’ fluorophore tag. The underlined sequence represents the 17 nucleotides that allow size discrimination between the BRAF V595 wild-type and mutant alleles. The lower case nucleotide represents the additional sequence mismatch introduced into the forward primer for the mutant allele to prevent spurious binding with the wild-type allele. The last two columns indicate the expected amplicon size obtained from wild-type alleles, and the observed amplicon sizes from the study cohort.
(XLSX)