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Genotyping amplified fragment length polymorphism marker ch4_0.68M and SNP marker 56900 in the parental and F2 generations.

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Figshare2020-06-16 更新2026-04-28 收录
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https://figshare.com/articles/dataset/Genotyping_amplified_fragment_length_polymorphism_marker_ch4_0_68M_and_SNP_marker_56900_in_the_parental_and_F_sub_2_sub_generations_/12491927
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The forward and reverse primer sequences of ch4_0.68M for genotyping using PCR are 5′-caattgcctgtttgtcagttacac-3′ and 5′-cgcctaatgccactccagcac-3′, respectively. Their locations are 685055–685078 bp and 685136–685156 bp on chromosome 4, respectively. The sizes of the amplified fragments separated using Microchip Electrophoresis System MCE®-202 MultiNA microchip electrophoresis (Shimadzu Corporation, Kyoto, Japan). (XLSX)
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2020-06-16
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