Characterization of the recombinant viruses obtained by reverse genetics.
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aThree independent plasmid transfections were performed for each construct followed by passaging in BHK-21 cells.bTime (h) when CPE was detected after transfection.cThe tag inserts were determined by RT-PCR and sequencing analysis.dThe virus titer were determined on BHK-21 cells by calculating the 50% tissue culture infectious dose per ml (TCID50/ml).eND: not done.
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2015-12-02



