Metabolic atlas of mouse aging - MALDI

The .mat files contain raw MALDI mass spec imaging data for mouse kidney sections (4 biological replicates for each age/sex group, 2 replicates on each slide). These can be further viewed and analyzed with the open source software, Isoscope (https://github.com/xxing9703/Isoscope, doi: 10.1038/s41592-021-01378-y). The metadata file annotates each pixel in each slide with the age/sex group of the mouse. -- Data were used to generate Figures 7C-G, J-N and S18 A-K in the corresponding publication, "A metabolic atlas of mouse aging." Pilley et al. Cell Metabolism, 2025. From the raw .mat files included here, slide matrices were combined, and peak picking was conducted using the "Untargeted workflow" in Isoscope. Peaks were annotated within a 5 ppm m/z error range using a NEDC-specific library of metablites and adducts in addition to all metabolites detected by LC-MS in any of the mouse tissues. Peak intensities were normalized by total ion count (TIC). Empty pixels (zero TIC) were dropped. Cortex and medulla regions were annotated using Leiden clustering of TIC-normalized data. Leiden clustering also identified artifact pixels (edge artifacts, tears), which were dropped. Combat was used to regress out the batch effect from slide-to-slide variation. The processed data are included in the supplementary data for the publication.