Selected PK parameters and clinical signs in ferret challenged with influenza B virus (Study 2).

a OP dose was calculated based on free-base oseltamivir molecular weight.b Values are mean ± SD. All PD parameters were from morning observations.c Each ferret’s body temperature was measured by subcutaneous implantable temperature transponders (Bio Medic Data Systems Inc., Seaford, DE, United States of America), and was recorded for 3 days before virus inoculation. The values were averaged to obtain a baseline value. The body temperature change was calculated for each ferret individually as an increase or decrease of its temperature on day 0 before virus inoculation. The values are averages for 5–6 animals per group (°C) ± SD. The average temperature decrease is indicated by minus. Positive average values indicate that temperature increased. The number of ferrets per the total number of animals in that group that had at least a 1°C increase in temperature from baseline is shown in parenthesis.d The weight change was calculated for each ferret individually as a percentage of its weight on day 0 before virus inoculation. The values are averages for 5–6 animals per group (%) ± SD. Positive average values indicate that animals were gaining weight. The number of ferrets per total number of animals in that group that had more than a 5% loss of initial body weight on day 5 p.i. is shown in parenthesis.e The reported value is the mean morning activity score ± SD observed on days 1–5 after inoculation on day 0. A score of zero indicated no nasal symptoms and a fully playful ferret. A score of one indicated nasal rattling or sneezing and a ferret that responded to play overtures but that did not initiate play. A score of two indicated nasal discharge and a ferret that was alert but not playful. A score of three indicated that mouth breathing was necessary and the ferret was not playful or alert.f These data are from the group of ferrets administered a different dose on day 1, 3, and 5 to obtain PK data.g This group is the uninfected PK control that received a 0.76, 3.8, and 19.0 mg/kg OP dose on days 1, 3, and 5, respectively.h Blood samples were not collected as OP was not administered.AUCss12h = steady-state 12-h area under the plasma concentration-time curve calculated from the population PK model individual fit CL for each animal: AUCss12h = D×CL; CL = clearance; OC = oseltamivir carboxylate; OP = oseltamivir phosphate; PD = pharmacodynamic; PFU = plaque forming units; p.i. = post-infection; PK = pharmacokinetic, SD = standard deviation.Selected PK parameters and clinical signs in ferret challenged with influenza B virus (Study 2).