CCDC88B affinity proteomic analysis

The CCDC88B gene (11q13) is a risk factor for several chronic inflammatory diseases in humans, including inflammatory bowel disease (IBD), multiple sclerosis and psoriasis. A Ccdc88b mutation in mice conveys protection against pathological inflammation in experimental models of microbial (cerebral malaria) and autoimmune (experimental autoimmune encephalomyelitis) of neuroinflammation and colitis. At the cellular level, loss of Ccdc88b function is phenotypically expressed as a migratory defect in vivo, and a cell mobility defect in vitro in mutant dendritic cells and lymphocytes. At the molecular level, CCDC88B belongs to a family of protein scaffold associated with the cytoskeleton and that feature protein:protein interactions domains. We used a co-immunoprecipitation / mass spectrometry-based approach to identify physical interactions of CCDC88B from mouse thymocytes and BI-141 cells.