Tcf1 and Lef1 regulate follicular helper T cell function eliciated by vaccination [ATAC-Seq]

The goal of this study is to determine the impact of Tcf1 and Lef1 deficiency on T follicular helper (Tfh) cells elicited by protein immunization. We demonstrate that although phenotypic Tcf1/Lef1-deficient Tfh cells were able to develop in response to vaccination, they are functionally impaired. Mechanisc studies reveal the direct regulatory effect by Tcf1 and Lef1 on transcriptome and chromatin accessibility in vaccine-elicited Tfh cells. Analysis of chromatin accessibility in WT and Tcf1/Lef1-deficient Tfh cells eliciated by vaccination. Overall design: WT or WT or Tcf1/Lef1-deficient Tfh cells were sort-purified from the lymph nodes, treated with TN5 transposase to generate tagged DNA fragments.