Hepatic and intestinal expression profile of genes involved in bile salt metabolism.

Hepatic and intestinal gene expression determined by quantitative real-time PCR of genes involved in synthesis, transport and signaling of bile salts in chow- and 0.5% cholic acid-fed PBS-injected and stably transduced scAAV-FXRα2 or scAAV-FXRα4 FXR knock-out mice. Gene expression levels were normalized to 36b4 and corrected to expression levels of PBS-injected FXR knock-outs. Values are presented as average ± standard deviations (n = 5–6 animals per group). *p<0.05 vs. PBS; #p<0.05 between FXR isoforms. ** note that our QPCR primers pairs always picked up FXR signal, even in FXR knock-outs. Expression levels were normalized to expression levels found in PBS-injected FXR knock-out mice.
Hepatic and intestinal expression profile of genes involved in bile salt metabolism.