Oxygen consumption rates in FM mitochondria from SF and BF <i>A. aegypti</i> along the blood digestion process oxidizing pyruvate and proline.

Oxygen consumption was determined in SF and in BF insects five different times ABM (0.25 h, 2 h, 24 h, 40 h and 72 h). Values were expressed as mean ± SEM of nmols oxygen consumed/min/mg protein in four different mitochondrial metabolic states using: 10 mM pyruvate + proline, followed by the addition of 1 mM ADP (ADP), 4 µg/mL oligomycin (Oligo) and 5–7 µM FCCP. RCR (respiratory control ratios) were calculated by dividing the rates of oxygen consumption in state 3 (ADP-stimulated) by those of state 4 (in the presence of oligomycin). Inhibition of oxygen consumption promoted by BF was expressed as the percentage relative to their respective metabolic states in SF (% red). Statistical analyses between groups were performed by using ANOVA and a posteriori Tukey's test. a, p<0.05; b, p<0.01; c, p<0.001 all relative to their respective metabolic states in SF.