Role of rsc2 in nucleotide excision repair in yeast

Nucleosomes are a significant barrier to the repair of UV damage because they impede damage recognition by nucleotide excision repair (NER). The RSC chromatin remodeler functions in cells to promote DNA access by moving or evicting nucleosomes and has been linked to NER in yeast. Here, we report genome-wide repair maps of UV-induced cyclobutane pyrimidine dimers (CPDs) in yeast cells lacking Rsc2. Overall design: UV-induced CPD lesions were mapped in yeast cells both Immediately after UV damage formation (0hr time point) and following two hours of repair (2hr time point) using the CPD-seq method. Repair of CPD lesions was measured in yeast cells mutated for the rsc2 subunit of the RSC ATP-dependent chromatin remodeling complex.