RNA-seq of murine proliferating neural stem and progenitor cells from Kat7-deleted mouse fetuses

Tissue-specific deletion of the gene encoding the histone acetyltransferase Kat7 (Hbo1) in the developing mouse central nervous system using cre-recombinase expression driven by regulatory sequences of the nestin locus (NesCre transgene) resulted in defective cerebral cortex development, a complete failure of neural stem cells to give rise to neurons and oligodendrocytes during in vitro differentiation, and a failure to express the neuronal differentiation program. Proliferating neural stem and progenitor cells (NPSCs) were isolated from Kat7-deleted and Kat7-intact 14.5 day embryonic mouse fetuses. RNA-seq profiling was undertaken of NSPC isolates from four Kat7-deleted (Kat7lox/loxNesCreT/+) and four Kat7-intact (Kat7+/+NesCreT/+) E14.5 fetuses. For completeness, the litter number that each embryo was obtained from was also recorded.