Selected curcuminoid effects and binding parameters in different cell lines.

1Doubling time with standard deviation (S.D.) of cells grown in DMEM with 5% fetal calf serum.2Maximum specific cellular binding of curcuminoids (BMAX) determined from the respective binding curves and presented as the amount bound to cells (nmol) per mg of total cellular protein with standard error (S.E.).3Determined with SOLID-solubilized curcuminoids. The values indicate the required minimal initial concentrations of curcuminoids in the media to achieve 100% cell death within 4 d of incubation with media changes every 24 h. Cellular senescence was assessed after 6 days of incubation.41,7-bis(4-hydroxy-3-methoxyphenyl)-5-hydroxy-1-heptene-3-one [CUR metabolite, C*]. 1-(4-hydroxy-3-methoxyphenyl)-7-(4-hydroxyphenyl)-5-hydroxy-1-heptene-3-one [DMC metabolite, D*]. 1,7-bis (4-hydroxyphenyl)-5-hydroxy-1-heptene-3-one [BDMC metabolite, B*].