Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and MttpIKO liver Transcriptomes on methioine defcient diet (MCD). Next Generation Sequencing Facilitates Quantitative Analysis of Wild Type and MttpIKO liver Transcriptomes on methioine defcient diet (MCD)

Purpose: The goals of this study is to use NGS-derived liver transcriptome profiling (RNA-seq) to identify candidates of pathways and genes which are responsible to protect MttpIKO from MCD induced-NASH development . Overall design: Mttpf/fVillin Cre mice were injected with Tamoxifen or vehicle and Keep on MCD diet for 3 weeks. Livers were collected after 4 hours fasting. Total liver RNA were extracted by TriZol reagent and 3 RNA pools (from 12 liver RNA) by genotype were submited for NGS.