H3K79me2 ChIP-seq in cerebellar granular neurons (CGN) and progenitors (CGNP) upon DOT1L inhibition with SGC0946 in vitro. Mus musculus

DOT1L as methyltransferase of H3K79 is implicated in brian development. Here, we further defined DOT1L function within the granular neurons during cerebellar development using ChIP-seq of H3K79 dimethylation of isolated cerebellar granular neurons and progenitors. Thereby we compared samples treated with a DOT1L inhibitor versus DMSO treated cells. The data sets reveals new important targets of DOT1L, which ensure a correct development of the cerebellum. Overall design: H3K79me2 ChIP-seq of CGNPs,4 h after DOT1L inhibition and CGNs, 44 h after DOT1L inhibition; compared to DMSO control each. Cells derived from murine cerebellum of P7 NMRI WT mice