The contribution of lipid droplets formation-related genes during adipogenesis to osteoblastogenesis

We tried to find out the contribution of lipid droplets formation-related genes during adipogenesis to osteoblastogenesis through RNA-Seq technique. Among the seventy-nine documented genes, APOB (apolipoprotein B), CES1 (carboxylesterase 1), and CIDEC (cell death inducing DFFA like effector c) were differentially expressed during osteoblastogenesis. We further confirmed gene function of APOB during osteoblastogenesis using lentivirus vector system. This study would provide the vital clues and molecular targets for balance regulation of hMSCs differentiation and new treatment strategy in metabolic bone diseases. Examination of 4 different sampling time during adipogenesis and osteoblastogenesis from human mesenchymal stem cells.