Micro-array analysis of the effects of a stable RORα over-expression on gene expression in HepG2 cells.

HepG2 cells were stably transfected with the pCMX-hRORα1 expression vector or with the pCMX insertless vector as a control. Total RNAs were extracted and mRNA levels were compared between these two experimental conditions with Agilent micro-arrays. This analysis revealed several genes whose expression was up-regulated or down-regulated by at least 2 fold in cells over-expressing RORα as compared to control cells. The results relative to sequences corresponding to known genes are shown. The gene symbols, gene description, genbank accession numbers and feature numbers on micro-arrays are given in columns 1 to 4. Gene expression levels in RORα over-expressing cells are expressed relatively to those in control cells. Results are given in column 5 as fold changes and in column 6 as the means of these fold changes +/− S.E.M. (n = 3 independent pools of transfected cells). Pvalues generated by the micro-array analysis are shown in column 7. It refers to the statistical significance of the differences between cells over-expressing RORα and control cells (all Pvalues are less than 0.01 except one over three for AGRP). Results corresponding to one spot/Feature Number representative of two are shown for PLG.