single cell analysis of PBMCs and kidney cells from SSc patients. Homo sapiens

To reveal immune cell abnormalities underlying the clinical heterogeneity of systemic sclerosis (SSc), peripheral blood mononuclear cells (PBMCs) were obtained from blood samples of 21 patients with SSc and 6 healthy donors. Kidney cells and whole white blood cells were obtained from a patient at the onset of scleroderma renal crisis. We measured single-cell transcriptomes and surface protein level of PBMCs using 10x chromium system (CITE-seq). We also measured single-cell transcriptomes and surface protein level of kidney cells and whole white blood cells using BD rhapsody system (Abseq). The Data Access Committee of the Database Center for Life Science (DBCLS) approved that this personal data was made published according to the NBDC Guidelines for Human Data Sharing (https://humandbs.dbcls.jp/en/guidelines/data-sharing-guidelines) as the NBDC Research ID hum0416 and the application ID J-DS000951-003.