Stringent and reproducible tetracycline-regulated transgene expression by site-specific insertion at chromosomal loci with pre-characterised induction characteristics-0

<b>Copyright information:</b>Taken from "Stringent and reproducible tetracycline-regulated transgene expression by site-specific insertion at chromosomal loci with pre-characterised induction characteristics"BMC Molecular Biology 2007;8():30-30.Published online 10 May 2007PMCID:PMC1884169.ch. B) The Screen and Insert type 1 (ScIn-1) strategy. C) The Screen and Insert type 2 (ScIn-2) strategy. Promoterless cassettes are shown for a generic gene of interest (GOI), and for genes encoding green fluorescent proteins (EGFP and d2EGFP), luciferase (luc), hygromycin phophotransferase (hygro) and guanosine phospribosyltranferase (gpt). See text for details.