Role of RccR in the transcriptional control of primary carbon metabolism in Pseudomonas. Role of RccR in the transcriptional control of primary carbon metabolism in Pseudomonas

To characterise the RccR regulon, we performed a ChIP-seq assay using a polyclonal RccR antiserum on SBW25 WT/ΔrccR strains grown in minimal pyruvate and glycerol media. We were able to identify 8 RccR binding sites from this experiment including one in the rccR promoter region itself. The peaks identified in our RccR ChIP-seq assays corresponded to strongly enriched regions relative to the respective ΔrccR controls. All 8 peaks were localized in intergenic regions, upstream of one or more genes.