Data underlying the publication: Geographical location drives microbial community diversity, B-complex vitamin enrichment, and functional properties during mabisi fermentation

The data were collected from a field survey which involved a systematic sampling of milk samples following traditional dairy fermentation in Zambia. The aim was to determine if geographical location influences the fermenting microbial community diversity and its community functionality, represented by B-complex vitamins, volatile organic compounds, pH and consistency (viscosity). The Illumina platform was used for 16S rDNA sequencing to profile the microbial communities, while high pressure liquid chromatography was used for B-group vitamin analysis, gas chromatography mass spectrophotometry was used for volatile organic compound determination, a pH meter for pH readings, and an Adam's consistometer for measuring the consistency.

本数据集的数据源自一项实地调研，该调研针对赞比亚传统乳制品发酵工序中的牛乳样本开展系统抽样采集。本研究旨在探究地理位置是否会影响发酵过程中的微生物群落多样性及其群落功能，其中群落功能以B族维生素、挥发性有机化合物、pH值及稠度（黏度）作为表征指标。测序分析采用Illumina测序平台（Illumina）进行16S核糖体DNA（16S rDNA）测序以解析微生物群落组成；B族维生素含量检测采用高效液相色谱法；挥发性有机化合物测定采用气相色谱-质谱联用法；pH值检测使用pH计；稠度测定则采用Adam氏稠度仪。