five

Snail and Twist expression in HMLE and MCF10A cells

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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE143349
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Snail and Twist are two EMT inducer, expression of Snail or Twist will induce EMT in HMLE and MCF10A cells. By introducing Snail or Twist in HMLE and MCF10A cells, which lack the expression of these two proteins, will identify the genes are induced during EMT. We used microarray analysis to compare the gene expression profiles between the mammamry epithleial cells and the cells undergone EMT. Expressing Snail or Twist in HMLE and MCF10A cells, stable clones were selected and RNA were prepared for microarray analysis
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2020-03-09
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