Genome wide CRISPR screen for Venetoclax resisatnce in MOLM13 cells using Brunello library

MOLM13 cells were infected with CRISPR/Cas9 library, selected for puromycn resistance for integration events and exposed to Venetoclax or vehicle, DMSO, at 1microMolar concentrations. Cells were collected at time 0 (post puromycin selection), day 7 and 14 , DNA was extracted and sgRNA barcodes were amplified. PCR library was deep sequenced using highthroughput Illumina platform Hiseq using 6 samples per lane. Overall design: perform pairwise comparisons between sgRNA distributions at initial time point (DMSO day 0) and plasmid, between DMSO and Venetoclax treated cells at corresponding time points.