The profound effect of ubiquitin-activating enzyme 1(TgUAE1) on the protein expression of Toxoplasma gondii by 4D label-free quantitative proteomics

After conditionally knocking down the putative ubiquitin activating enzyme TgUAE1 in Toxoplasma gondii, a series of cutting-edge techniques, including protein extraction, enzymatic digestion, liquid chromatography-mass spectrometry (LC-MS/MS) analysis, and bioinformatics analysis, were synergistically integrated to investigate the quantitative proteome of the samples and identify potential target proteins of TgUAE1