Exon Level Expression Profiling: a Novel Unbiased Transcriptome

Transcriptome analysis of partially degraded and fragmented RNA samples from hiPSCs-derived MNs with Venus or Fos-B + Venus expression by lentivirus infection Fos-B could had a function on axon branching with hiPSCs-derived MNs. However, the grobal profiling under Fos-B expression was not fully elucidated. Thus, we constructed Venus or Fos-B + Venus expression lentivirus and subsequently infected those virus to hiPSCs-derived MNs. Increasing number of axon branching was detected with Fos-B expression MNs, and we demonstrated the pathological confirmation of true exon level expression profiling approach with Fos-B expressed MNs. We analyzed hiPSCs-derived MNs with Venus or Fos-B + Venus expressing lentivirus infection each from 3 baches using the Affymetrix Human Exon 1.0 ST platform. Array data was processed by Affymetrix Exon Array Computational Tool. No techinical replicates were performed.