gut microbiota and ventilator-associated pneumonia. undefined

The aim of this study is to assess the link between gut microbiota composition and subsequent occurrence of ventilator-associated pneumonia in critically ill influenza patients. Every patient admitted to intensive care unit from October 2019 to March 2020 who received at least 2 days of invasive mechanical ventilation were included (ancillary study of Microbe study, NCT04131569). Rectal swabs were sampled and DNA extraction was performed by QIAamp® PowerFecal® Pro DNA kit (QIAgen®). V3-V4 regions of 16SRNA coding gene was amplified to analyse the bacterial kingdom of lung microbiota (bacteriobiota) and ITS2 to analyse the fungal kingdom (mycobiota). Sequencing (2x250bp paired-end reads) was performed on MiSeq sequencer (Illumina®) and DADA2 pipeline on R software was used for bioinformatics analyses.