whole-genome sequencing of HR1212 and 41 breeding materials

Huruan1212 (HR1212) is well-regarded for its superior eating and cooking quality in the lower reaches of the Yangtze River in China. Still, its high susceptibility to rice blast and lack of fragrance have limited its further spread and utilization. In this study, we utilized a molecular marker-assisted selection backcrossing strategy to introduce Pigm, Pi-ta, and badh2 into introgressed lines employing re-sequencing for precise genetic background selection. This study expanded the germplasm resources of japonica, providing a material foundation for enhancing breeding programs aimed at developing rice blast-resistant and high-quality fragrant japonica varieties. Additionally, the study demonstrated that integrating molecular markers and re-sequencing can inform breeders' decision-making more precisely and efficiently.