A refined, minimally invasive, reproducible ovine ischaemia-reperfusion-infarction model using implantable defibrillators: methodology and validation

This project sought to develop a translationally relevant preclinical model of myocardial infarction. We developed a sheep model using minimally invasive surgical approaches utilising standard clinically available devices and equipment. The developed model was associated with a marked reduction in overall mortality compared to other studies, having at least a 50% decrease in mortality rate when compared to the best performing similar studies. The associated publication provides a detailed methodological approach. The model satisfies key criteria of the 4th Universal Definition of Myocardial Infarction and provides a platform for future mechanistic and therapeutic assessment studies.What is the central question of this study?· We sought to develop and describe in detail a minimally invasive, reproducible large animal model of myocardial ischaemia-reperfusion-infarction with improved survival outcomes and high translational relevance.What is the main finding and its importance?· Using minimally invasive balloon angioplasty approaches we developed a reproducible mode of ischaemia-reperfusion-infarction in sheep which satisfies key criteria in the 4th Universal Definition for Myocardial Infarction.Using an implantable cardiac defibrillator and intra-surgical antiarrhythmic drug routine, intraoperative mortality was reduced by at least 50% when compared to other large animal models of myocardial infarctionUnderpinning data is included where each Tab in the excel book indicates appropriate figure panels

本课题旨在构建一种具有翻译学相关性的心肌梗死临床前模型。通过采用微创外科手术方法，并利用标准临床可用的设备和器械，我们建立了一种绵羊模型。与既往研究相比，该模型显著降低了总体死亡率，其死亡率至少降低了50%。相关出版物详细介绍了方法论。该模型满足了第四版《心肌梗死通用定义》的关键标准，并为未来机制和治疗效果评估研究提供了平台。本研究的核心问题是什么？我们旨在详细描述并开发一种微创、可重复的大型动物心肌缺血-再灌注-梗死模型，该模型具有改善的生存结果和高度的翻译学相关性。主要发现及其重要性是什么？通过采用微创球囊成形术方法，我们在绵羊中开发了一种可重复的缺血-再灌注-梗死模式，该模式满足了第四版《心肌梗死通用定义》的关键标准。通过植入式心脏除颤器和术中抗心律失常药物常规使用，与其它大型动物心肌梗死模型相比，术中死亡率至少降低了50%。数据支持文件包括Excel表格中的每个标签页，其中每个标签页都指示了相应的图示部分。