Total RNA from Wolbachia-infected Drosophila Jw18 cells. Wolbachia transcriptomic response to viral infection

Wolbachia-positive Jw18 cells were infected with Semiliki Forest Virus at an MOI of 20. 4 and 24 hours post infection cells were lysed in Trizol. Total RNA was extracted, treated with TURBO DNase and purified. Drosophila ribosomal RNA was then depleted using the Ribo-Zero Gold Magnetic kit (Human/Mouse/Rat, Epicentre). Libraries of the rRNA-depleted total RNA were prepared with the Truseq RNA Sample Preparation kit (Illumina) and sequenced in 4 lanes on a HiSeq 2000.