5' droplet-based single cell RNA sequencing of human colorectal cancer and normal organoids

Using 5' droplet-based single cell sequencing, we profiled single cells dervied from human colorectal cancer organoids carrying either APC mutation or RSPO fusion, and paired normal colon organoids for the later. Colorectal cancer and normal organoids were cultured in standard colon organoid medium or specified culture conditions to achieve differentiated state. Organoids were then FACS-sorted to obtain single cells and were subjected to sequencing. The raw data files have not been included due to privacy concerns.