Semi-quantitative distribution of CSF-injected CFSE⁺ DCs in the CNS of normal or EAE rats.

In parallel experiments, DCs labeled with the cytoplasmic fluorescent marker CFSE were injected intra-CSF to normal rats (n = 7) or EAE rats (n = 8). Serial brain sections crossing different brain areas (anterior brain, midbrain, brain stem and cerebellum) were then assessed for the presence of CFSE+ cells, on day 1 post-injection (EAE rats: n = 4, normal rats: n = 4). A semi-quantitative analysis was performed as follows: −: none; +/−: occasional cells; +: 2 to 5 cells per section; ++: 5 to 10 cells per section; +++: >10 cells per section. LV: lateral ventricle, V3: third ventricle, V4: fourth ventricle, SVZ: subventricular zone.