Deficiency of Rpsa results in reduced levels of H3K4me3 in inflammatory cytokines promoter regions

After HSV-1 infection, ribosomal protein RPSA can recognize viral DNA and promotes the expression of proinflammatory cytokines. Through ChIP-seq, we found that the level of H3K4me3 in proinflammatory cytokines(Il1b, Il6, Il12b, etc.) promoter regions was decreased due to Rpsa deficiency. We constructed Rpsa induced konckout RAW264.7 cell lines and performed chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for H3K4me3 in WT and Rpsa-iKO RAW264.7 cells infected with or without HSV-1.