RNA-seq profiles of pluripotent and surface ectoderm cells with and without p63

Purpose: Evaluate p63 regulation of gene expression in human embryonic stem cells and the surface ectoderm Methods: RNA profiles for human embyronic stem cells and surface ectoderm cells with and without p63 using deep sequencing, in duplicate, on Illumina NextSeq 500 sequencer. Quality of reads were checked by fastqc. Reads were aligned to hg19 genome using tophat and FPKM values were generated using Homer. Results: p63 is largely unable to regulate gene expression in pluripotent cells, but acts primarily as a repressor in the surface ectoderm. Conclusion: p63 regulation of gene expression is dependent on the changes the cell undergoes during surface ectoderm commitment RNA-seq profiles of human embryonic stem cells and surface ectoderm cells with and without p63 were generated in duplicate by deep sequencing on Illumina NextSeq 500 sequencer. ***Please note that the submitters no longer have access to the fastq or all of the original bam files (i.e. no raw data). The text files used for all downstream analyses were provided.