Glioblastoma stem cells RNAseq profiling

RNAseq-profile of independent glioblastoma stem cells lines was determined Glioma stem cells were derived from glioma specimens obtained after informed consent from patients with a median age of 62 years (range 48-79). Tumor samples were obtained during surgery from patients of the Neurosurgery Department, San Martino Hospital (Genova, Italy). All patients underwent surgery for the first time and never received chemotherapy or radiotherapy. Tumor samples, classified as gliomas grade IV based on World Health Organization criteria, were immediately processed for isolation of single cells by mechanical dissociation and plated at the concentration of 10e5 cells/ml in Matrigel-coated culture flasks.