Transcriptome analysis of shooting and root parts of diploid and tetraploid millet

Foxtail millet, as a self-pollinating diploid (2n = 2X = 18) crop, tetraploid research is scant. In foxtail millet research to date, an autotetraploid of ‘Jingu 21’ has only been obtained via colchicine-induced mutation. Relative to its diploid, this autotetraploid exhibits substantial divergence in the regulation of endogenous hormones pertaining to the morphogenesis of the main stem, along with pronounced disparities in the relevant physiological mechanisms.Concomitantly, certain phenotypic traits of the autotetraploid millet surpass those of the diploid. Apart from the utilization of colchicine to induce polyploidy in foxtail millet, there are currently no alternative methods established for achieving tetraploid states. Previous investigation in foxtail millet has documented and confirmed the morphological, proteomic, and temporal network in response to drought. However, no studies have yet compared the transcriptomic changes in response to drought among different ploidy levels of millet.We took the shooting and root parts of diploid and tetraploid 24 h after PEG treatment for transcriptome sequencing.