Comparison of three methods used to measure serum levels of HSV-2-specific antibodies.

aRange of HSV-2 antiserum dilutions in which estimates of anti-HSV-2 antibody abundance changed in linear relation to changes in serum dilution.
bMean ± sem coefficient of variation of triplicate measurements for each serum dilution in the linear range of each assay. For each serum dilution considered, the coefficient of variation = 100× standard deviation ÷ mean.
cGoodness-of-fit (r2) of observed data relative to values predicted by a regression model within the linear range. The p-value refers to the probability that the quantity measured by each assay (i.e., neutralizing titer, OD405, or ΔMFI) did not vary as a function of HSV-2 antiserum dilution.