Identification of the Drosophila centriolar satellite proximity interactome by direct and indirect biotin proximity labeling

In this set of experiments we identified the main scaffolding component of centriolar satellites, PCM1 or Cmb in Drosophila as a proximity interactor of the centriolar structural protein Sas4 by BioID in S2 cells and subsequently explored the Cmb proximity interactome by direct TurboID in S2 cells and indirect, GFP nanobody-targeted, TurboID in Drosophila testes.