_A_AgroinfFinalConstructsdCas9EV-GCMS.output.raw

pISA-tree file path: _p_SUSPHIRE\_I_T12_CuInducible\_S_P1_TransientExp_Nb\_A_AgroinfFinalConstructsdCas9EV-GCMS\output\raw Title: Agroinfiltration of final constructs for copper-inducible pathway in Nicotiana benthamiana leaves Description: Two constructs were made containing the three genes of the moth pheromone pathway under the regulation of synthetic promoters made in T11, nptII selection marker and with/without the gRNA1 for activation. In parallel, another construct containing the copper-inducible dCas9EV system with/without the guide was made in pLX vector so it could be co-transformed in the same agrobacterium colony with the moth pheromone pathway. Such Agrobacterium containing both plasmids (guided/nonguided pathway + nonguided/guided copper-inducible dCas9EV) was agroinfiltrated before stable transformation in Nicotiana benthamiana to check the expression of the genes.