Effect of cytokines on human myoblast or myotube cultures.

A) LHCNM2 human skeletal muscle myoblasts were cultured under differentiation conditions for the indicated number of days in the presence or absence (−) of the indicated cytokines. Cell lysates prepared on the indicated day were analyzed by immunoblotting for the protein indicated at the left. B) Experiment as in A with addition of indicated cytokines on differentiation day 8 when myotubes had formed. Molecular mass (kilodaltons) of the proteins detected is indicated at the right.