Disruption of the Integrator complex triggers the Integrated Stress Response through formation of double-stranded RNA [TTseq]

Acute loss of INT induces transcription factors that initiate stress-responses like the Integrated Stress Response (ISR). Inefficient elongation upon INT loss enables termination in introns and dsRNA formation. dsRNA is recognized by PKR, which persistently activates the ISR in samples from patients with INT mutations Overall design: TT-seq performed after 10' labelling in presence or absence of acute degradation of INTS1.