Angiotensin II Increases Oxidative Stress and Inflammation in Female, But Not Male, Endothelial Cells

To investigate sex differences in endothelial cell response to AngII, male and female human umbilical vein endothelial cells (HUVEC) were treated with AngII for 24 hours. RNA seq was run, and demonstrated that female HUVEC had elevated genes associated with oxidative stress and inflammatory responses compared to male HUVEC following AngII treatment. Male and female HUVEC (pooled from 3 donors per sex) were treated with 1 uM AngII for 24 hours before isolating RNA and running RNA sequencing