α-Ketoglutarate inhibits the pluripotent-to-totipotent state transition

In early mouse embryogenesis, the distinct enrichment of α-ketoglutarate (αKG) in blastocysts and L-2-hydroxyglutarate (L-2HG) in 2-cell (2C) embryos serves as a key metabolic signature. While elevated L-2HG levels inhibit the resolution of totipotency during the transition from the 2C stage to the blastocyst, the role of αKG remains elusive. Mouse embryonic stem cells (mESCs) cultured in vitro naturally harbor a subpopulation that transitions dynamically into a 2C-like totipotent state, providing a convenient model to investigate the role of αKG in totipotency reprogramming. This study demonstrates that αKG significantly inhibits the pluripotency to totipotency transition through upregulating ten-eleven translocation (TET) DNA hydroxylases. We further show that reducing endogenous αKG levels via glutamine withdrawal or inhibiting αKG-dependent dioxygenases by blocking succinate dehydrogenase (SDH) markedly enhances the induction of 2C-like cells (2CLCs). Finally, leveraging the potent ..., , , # README: Supplementary Data Description

## General Information

This README file provides details about the supplementary tables included in the dataset. These tables contain shRNA sequence information, primer details,  RNA sequencing, and scRNA sequencing data related to MERVL-GFP cell line under different experimental conditions.

## File Description

The provided Excel file consists of the following supplementary tables:

### **Supplementary Table 1: shRNA Sequences**

* This table contains the sequences of short hairpin RNAs (shRNAs) used in the study.
* Column descriptions:
* **Gene**: Gene targeted by the shRNA
* **Target Sequence**: Nucleotide sequence of the shRNA

### **Supplementary Table 2: Primer Sequences for RT-qPCR**

* This table lists the primer sequences used for reverse transcription quantitative PCR (RT-qPCR).
* Column descriptions:
* **Gene:** Target gene for qPCR
* **Forward Primer (5'→3')**: Forward primer sequence
* **Reverse Primer (5'→3')**: Revers...