Next Generation Sequencing of CNS-infiltrating Tox-sufficient and -deficient OT-1 cells in ODC-OVA mice 7 days after LCMV-OVA infection

Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goal of this study is to compare the transcriptome of CNS-infiltrating OT-1 WT and Tox-deficient cells during effector phase (7 days after infection with LCMV-OVA) OT-1 mRNA profiles of 7-weeks old ODC-OVA mice adoptively transfered with 10^5 OT-1 CD45.1 cells (WT and Tox-deficient) 7 days after i.v. infection with LCMV-OVA (deep sequencing, in triplicate, using Illumina).