Transcriptomic Profiling of the Telomerase Transformed Mesenchymal Stromal Cells Derived Adipocytes in Response to Rosiglitazone.

In our earlier study, it was shown that Rosiglitazone enhances the brown adipogenesis of Immortalized Human Bone Marrow Mesenchymal Stromal Cells - hTERT (iMSC3). The current study compared the transcriptomics profiles of the different adipocytes derived from iMSC3 reported in our previous study. The complete transcriptomic profiles of iMSCs and adipocytes differentiated with and without Rosiglitazone treatment revealed a set of marker genes that warrant further investigation. The transcriptomics analysis revealed the upregulation and uniquely expression of genes due to Rosiglitazone treatment seems to have roles in brown adipogenesis. Total RNAs were isolated from iMSC3 and iMSC3‑derived adipocytes without and with Rosiglitazone treatment. The experminamtal groups were as following: (A) undifferentiaed iMSC3; adipocytes (B) derived with no Rosiglitazone treatment; adipocytes (C) derived with Rosiglitazone present in induction media only while adipocytes (D) derived with Rosiglatazone present in both induction and maintenance media. This was followed by library preparation for transcriptomic sequencing. The constructed libraries were subjected to high throughput mRNA sequencing using Illumina NovaSeq 6000. Differential gene expression analysis was performed using DESeq2 R package, followed by GO and KEGG enrichment analysis using clusterProfiler R package.