Transcription profiling of mouse liver from long lived Prop-1 mutant (Ames) vs. wild type controls

To compare and contrast genetic signatures from livers of young and aged Ames dwarf mice with their wild type controls. SUBMITTER_CITATION: W. H. Boylston, James H. DeFord, John Papaconstantinou (2006) Identification of longevity-associated genes in long-lived Snell and Ames dwarf mice Age 28:125-144 Experiment Overall Design: 25 micrograms of liver RNA was isolated from young (5 dwarfs, 5 wildtype), middle aged (5 dwarfs, 5 wild type) and aged (5 dwarf, 6 wild type) mice for use in hybridization of Affymetrix gene chips according to Affymetrix protocols.本研究旨在对比年轻、老年Ames侏儒小鼠（Ames dwarf mice）及其野生型对照小鼠的肝脏基因特征。 提交者引用：W. H. 博伊尔斯顿（W. H. Boylston）、詹姆斯·H·德福德（James H. DeFord）、约翰·帕帕康斯坦蒂努（John Papaconstantinou）（2006年）《长寿型Snell侏儒小鼠（Snell dwarf mice）与Ames侏儒小鼠的长寿相关基因鉴定》，《Age》28卷：125-144页 实验总体设计：分别从年轻组（5只Ames侏儒小鼠、5只野生型小鼠）、中年组（5只Ames侏儒小鼠、5只野生型小鼠）以及老年组（5只Ames侏儒小鼠、6只野生型小鼠）的小鼠体内提取25微克肝脏RNA，依照Affymetrix标准实验规程，完成Affymetrix基因芯片杂交实验。